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BT:猪粪厌氧消化过程中抗性基因归趋影响因素研究-抗生素与抗性基因

BT:猪粪厌氧消化过程中抗性基因归趋影响因素研究-抗生素与抗性基因
Abstract:This study investigated the fate of antibiotic resistance genes (ARGs) during mesophilic (mAD) and thermophilic digestion (tAD) of swine manure through zero valent iron (ZVI), natural zeolite and Dnase addition. Changes of microbial community, intI1, heavy metal resistance genes (MRGs) and virulence factors (VFs) were followed to clarify the influencing factors to ARGs reduction. Results showed that AD could realize ARGs reduction with tAD superior to mAD快乐学围棋 , and ZVI and natural zeolite could further enhance the reduction, especially for natural zeolite addition at mAD. The reduction efficiency of the relative abundance of ARGs was increased by 33.3% and 138.5% after ZVI and natural zeolite addition, respectivelykaix, but Dnase deteriorated ARGs reduction at mAD. Most of ARGs could be reduced effectively except sulII and tetM. Network analysis and partial redundancy analysis indicated that co-occurrence of MRGs followed by microbial community contributed the most to the variation of ARGs fate among treatments.
中文摘要:本研究通过零价铁(ZVI),天然沸石和Dnase添加剂研究了猪粪肥在嗜温(mAD)和嗜热消化(tAD)过程中抗生素抗性基因(ARG)的命运。翟山鹰依据微生物群落楚汉争雄,intI1,重金属抗性基因(MRGs)和毒力因子(VFs)的变化来阐明ARGs减少的影响因素。结果表明钱永刚 ,AD可以实现ARGs的还原,其中tAD优于mAD,ZVI和天然沸石可以进一步促进还原宁远房产网 ,尤其是在天然沸石添加时。添加ZVI和天然沸石后,ARGs相对丰度的降低效率分别提高了33.3%和138.5%张劲东 ,但是Dnase降低了mAD时ARGs的降低。除了sulII和tetM之外sp训诫文,大多数ARGs可以有效地减少。网络分析和部分冗余分析表明,MRGs与微生物群落共同发生对处理中ARGs命运的变化贡献最大。(Google翻译史小鹏 ,未整理田菜农 ,供参考)
DOI:10.1016/j.biortech.2018.03.005